Sub-cellular biopsy: a key technology to gather fundamental knowledge for effective cancer therapy

Posted by Erasmus MC/TU Delft 115 days ago

We are looking for two self-motivated postdocs with a strong interest in working in a flagship multidisciplinary project between Erasmus MC and TU Delft on sub-cellular biopsy, supported by the Convergence initiative. The goal of this project is to enter the nucleus of cells using microfluidic atomic force microscopy cantilevers and to extract temporally formed organelles involved in repair of DNA damage for subsequent analysis by cryo-EM and mass spectrometry. One aspect of the project involves fabrication of the cantilevers to be used to interrogate individual cells by harvesting the sub-cellular organelles and their components. Another aspect is the generation by genomic engineering of appropriate cell lines expressing fluorescent DNA repair proteins in different genetic backgrounds to dissect distinct steps in DNA repair. The project will be executed in a multi-disciplinary team from Erasmus MC and TU Delft consisting of experts in Molecular Genetics, Cell Biology, (Bio)mechanical Engineering, Microfabrication, Instrumentation, Electron Microscopy and Mass Spectrometry.

Qualifications and skills

Postdoc position 1:

  • PhD in Physics/Applied Physics/Mechanical Engineering/Biomechanical Engineering/Micro-Nanotechnology

Skills desired:

  • Atomic force microscopy
  • Microfluidics
  • Microfabrication
  • Biomechanical characterization
  • Building measurement schemes for micro-nanoscale devices
  • Characterization methods like SEM, TEM, mass spectrometry
  • Collaborative and team player

Postdoc position 2:

  • PhD in Molecular Biology/Biochemistry

Skills desired:

  • Recombinant DNA techniques
  • Tissue culture
  • Live cell fluorescent microscopy
  • Mass spectrometry analysis
  • Collaborative and team player

Expectations 

The postdocs should have a deep interest in the convergence of health sciences, physical sciences, and engineering to foster individual health and keep the healthcare system sustainable. They will work closely with the supervisors, Principal investigators and Collaborating investigators of the project to conduct high calibre research. They are also encouraged to initiate and lead collaborative work with other postdocs within the Convergence for health(care) initiative. Salary will be commensurate with qualification and experience.

About this position

Recruitment takes place until the vacancy is filled.
Hours a week:

Contact information:

Prof. Roland Kanaar
Erasmus MC
r.kanaar@erasmusmc.nl
Murali Ghatkesar
TU Delft
m.k.ghatkesar@tudelft.nl

Interested?

Sub-cellular biopsy: a key technology to gather fundamental knowledge for effective cancer therapy